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| Dias,Renata Gonçalves; Sampaio,Sandra Coccuzzo; Sant’Anna,Morena Brazil; Cunha,Fernando Queiroz; Gutiérrez,José María; Lomonte,Bruno; Cury,Yara; Picolo,Gisele. |
| Abstract Background Arthritis is a set of inflammatory conditions that induce aching, stiffness, swelling, pain and may cause functional disability with severe consequences to the patient’s lives. These are multi-mediated pathologies that cannot be effectively protected and/or treated. Therefore, the aim of this study was to establish a new model of acute arthritis, using a Lys49-PLA2 (Bothrops asper myotoxin II; MT-II) to induce articular inflammation. Methods The articular inflammation was induced by MT-II (10 μg/joint) injection into the left tibio-tarsal or femoral-tibial-patellar joints. Cellular influx was evaluated counting total and differential cells that migrated to the joint. The plasma extravasation was determined using Evans blue dye. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lys49-PLA2; Myotoxin II; Arthritis; Bothrops asper; Phospholipase. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100307 |
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| Calvete,Juan J.; Petras,Daniel; Calderón-Celis,Francisco; Lomonte,Bruno; Encinar,Jorge Ruiz; Sanz-Medel,Alfredo. |
| Abstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venomics; Top-down proteomics; Top-down venomics; Protein species-resolved venomics; Absolute quantification; Inductively coupled plasma mass spectrometry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100205 |
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| Lomonte,Bruno; Calvete,Juan J.. |
| Abstract This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Proteomics; Venomics; Antivenomics; Toxicovenomics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100206 |
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